Lung Cancer Cell Lines A549

To study the ability of 2 2 4 dihydroxyphenyl th ieno 1 3 thiazin 4 one bchtt to inhibit cancer cell proliferation and identify mechanisms involved in its actions to study the effects of insulin and insulin like growth factor 1 igf1 on proliferation and apoptosis in a549 cells.

Lung cancer cell lines a549.

All experiments should include physiologically relevant controls. Eml4 alk fusion a549 isogenic cell line human. Cell line history. Physiologically relevant controls.

Purchasing of cell lines and culture. Select lung cancer cell lines authenticated by ecacc based on associated mutations and diagnosis classifications. Human a549 cell line has been used. Today a large number of human non small cell lung cancer cell lines exist that are being used for both basic research and drug discovery.

Human lung cancer cell lines a549 was acquired from national center for cell science nccs pune india. Through the removal and culturing of cancerous lung tissue in the explanted tumor of a 58 year old caucasian male. The cells were cultured in dmem medium supplemented with 10 fbs and with penicillin at 100 µ ml and streptomycin at the concentration of 100 µg ml were used as antibiotics. One commonly used line is a549 an epithelial carcinoma derived from a 58 year old male patient known to be kras mutant and egfr wild type.

Browse squamous cell adenocarcinoma sclc and nsclc cell lines for drug response toxicology in vitro models including a549 nci h292 and more. Lung cancer cell lines eml4 alk fusion a549 a549. Atcc provides both primary and htert immortalized. Despite being developed as a tool for cancer research the cell line was consequently characterized as being representative of the alveolar type ii pneumocytes of the.

The a549 cell line was isolated in 1973 from a pulmonary adenocarcinoma. Ten human non small cell lung cancer nsclc cell lines and samples from thirty patients with nsclc were analyzed for the expression of mir 29b by quantitative rt pcr. The a549 cdx model is one of the most used xenograft lung cancer models by researchers for studying soc standard of care and novel therapeutics i e. Tumor suppressive activity of the growth arrest specific gene gas1 in human tumor cell lines.

In a study to attempt to establish continuous cell lines from 200 different tumours. A549 cells are adenocarcinomic human alveolar basal epithelial cells and constitute a cell line that was first developed in 1972 by d. Our pilot study using mirna pcr array found that mirna 29b mir 29b is differentially expressed in primary cultured cd133 positive a549 cells compared with cd133 negative a549 cells. The cells are used as models for the study of lung cancer and the development of drug therapies against it.